Studies of human granulocytes function, especially in reference to infection
نویسندگان
چکیده
منابع مشابه
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1. A granulocyte-rich fraction was isolated from blood of a patient with chronic granulocytic leukaemia and from blood of a normal subject and the cells were disrupted in isotonic sucrose. The nuclei were removed by low-speed centrifugation and the post-nuclear supernatant was fractionated by centrifugation on sucrose density gradients. 2. The subcellular organelles in the gradients were detect...
متن کاملCryopreservation of Human Granulocytes : Study of Granulocyte Function
Human granulocytes can be cryopreserved with dimethyl sulfoxide (DMSO) at 80’C. However, the percent recovery of functional cells has been unsatisfactory to date. Throughout this study we have been able to prepare relatively pure granulocytes ( 85%). cryopreserve them with 5%-1O% DMSO with and without serum, and store them at -80’C for up to 4 mo. The parameters studied were absolute cell count...
متن کاملCryopreservation of human granulocytes: study of granulocyte function and ultrastructure.
Human granulocytes can be cryopreserved with dimethyl sulfoxide (DMSO) at--80 degrees C. However, the percent recovery of functional cells has been unsatisfactory to date. Throughout this study we have been able to prepare relatively pure granulocytes approximately equal to 85%), cryopreserve them with 5%--10% DMSO with and without serum, and store them at--80 degrees C for up to 4 mo. The para...
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ژورنال
عنوان ژورنال: Ensho
سال: 1981
ISSN: 0389-4290,1884-4006
DOI: 10.2492/jsir1981.1.65